Genomics and Bioinformatics Core

The Genomics and Proteomics Core at Yale Stem Cell Center, funded by the State of Connecticut and Yale University, supports stem cell research for research groups at Yale and in the State in gene expression experiments, transcription mapping analyses, and proteomic assays.

Equipment and Location

The core is located in the second floor of new Amistad building and Keck facility. It is in the process of establishing microarray processing, mass spectrometry, an shRNAi library, robotics, and bioinformatics components. For microarray processing, both Affymetrix and Nimblegen platforms will be supported. The workhorse of mass spectrometry is a Micromass Q-tof suitable for protein profiling and mapping posttranslational modifications. This instrument resides in the Keck facility. A Cellomics scanner is be used for cell-based screening assays and for screening of subcellular features. A qPCR machine serves to quantify gene expression and transcription factor binding as well as for validation of microarray results. The core plan to purchase from Open Biosystems an shRNAi library of >200,000 constructs cloned into a lentivirus vector and directed against human RNAs. The core also plans to establish bioinformatics service. This service will assist groups from the State of Connecticut in microarray design and analyses.

Core Management

The Genomics and Proteomics Core will be managed by Drs. Snyder and Weissman. If a large number of requests are received, they will be reviewed by an executive committee comprised of Drs. Michael Snyder, Sherman Weissman and Haifan Lin. It is our goal to provide everyone working on human ESCs access to the facilities and equipment. Investigators will be responsible for most supplies and arrays. Access to the mass spectrometer at the Keck Center will be on a fee-for-service basis.